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Here's the catch: MOTS-c levels decline with age

TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples

Nat Chem Biol 16(5):497506

Modern English references (Ashmarin 2010, Levitskaya 2004, Myasoedov 2017) provide bibliographic entry points to systematically deepen before designing a protocol, to avoid reinventing results already published 30 years ago in a less accessible language